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Laboratory

 

Dr. Mohammad Yaqoob

Associate Professor

yaqoob_chem@uob.edu.pk

yaqoob2001@hotmail.com

Research Interests

 

Analytical & Environmental Chemistry

Flow injection techniques (chemiluminescence, uv-visible & amperometry)

Enzyme isolation and purification                                                                                

Immobilized enzymes.

Membership/Distinctions

  • Life member of the Chemical Society of Pakistan.

  • Member of the Academic Council, University of Balochistan, Quetta (Dec. 2000 to Aug. 2004).

  • Technical referee to evaluate scientific research papers in national /international peer review journals.

  • Biographee in “Who’s Who in the World, 19th Edition USA, 2001.

  • HEC approved PhD supervisor, April 2005.

 
Projects Sponsored
  • TWAS 1993-1994                Biotechnological potential of immobilized enzymes for the production of biosurfactants, concluded.

  • UGC   1997-1998                Biochemical and analytical applications of immobilized enzymes, concluded.

  • TWAS  2000-2001               Flow injection chemiluminescent assays for biologically important analytes using immobilized enzymes, concluded.

  • PSF    2005-2007                Clinical applications of electrogenerated chemiluminescence detection using tris(2,2-bipyridine) ruthenium(II) with flow injection analysis." Approved for funding by Pakistan Science Foundation.

  • PSF    Jan. 2005              Bioanalytical potential of immobilized enzymes for the determination of Vitamin-A using flow injection with chemiluminescence detection, submitted.

Research Co-Supervision and Courses Taught

M.Phil and Ph.D. students are co-supervised on projects sponsored by the National and International Organizations. Teaching various courses of analytical Chemistry at post-graduate level.

Ph.D. Students (Co-supervisor)

  • Mr. Mohammad Anwar, Assistant Professor, Department of Chemistry, University of Balochistan, working on “Bioluminescent assay of ATP as a tool of quantitation of the viability of vaccines”.

  • Mr. Amir Waseem, Lecturer, Department of Chemistry, University of Balochistan, working on "Clinical applications of chemiluminescence detection using Tris(2,2-bipyridine) ruthenium(II) with flow injection analysis".

M.Phil. Students (Co-supervisor)

  1. Mrs. Shaheen Wali, Lecturer, Department of Chemistry, University of Balochistan, working on "Determination of amino acids by its inhibition effect on cobalt(II) catalyzed luminol chemiluminescence reaction".

  2. Mr. Abdul Rashid, Assistant Professor, Govt. College, Quetta worked on "Analytical applications of chemiluminescence to enzymatic methods of analysis" Degree awarded in 1998.

 

Publications

 

1.       M.Yaqoob, M. Masoom and A. Townshend, Flow injection procedures for the determination of iodide and iodate/iodide with spectrofluorimetric and spectrophotometric detection system respectively, Anal. Chim. Acta, 248 (1991) 219-24.

2.       M.Yaqoob, M. Anwar, A.S.Masood and M. Masoom, Flow injection method for sulphide determination using an organic mercury compound, Anal. Lett., 24 (1991) 581-88.

3.        M. Yaqoob, L.Rishi and M. Masoom, Comparative study of different methods for sulphide determination when adopted to flow system, J. Chem. Soc. Pak., 13 (1991) 32-38.

4.       M. Yaqoob, M. A. Siddiqui and M. Masoom, Spectrophotometric flow injection determination of nitrate and nitrite in potable water using 8-hydroxyquinoline, J. Chem. Soc. Pak., 13 (1991) 248-52.

5.         M. Yaqoob, M. A. Siddiqui and M. Masoom, Flow injection spectrophoto-metric determination of nitrite in water samples, J. Chem. Soc. Ind. 69 (1992) 32-33.

6.         M. Yaqoob and M. Masoom, UV-spectrophotometric flow injection determination of nitrate using on-line charcoal column, J. Chem. Soc. Pak., 14 (1992) 214-43.

7.         M. Yaqoob, M. Anwar and M. Masoom, Emulsified substrates in flow system: the determination of lipase activity, J. Chem. Soc. Pak., 16 (1994) 112-14.

8.         M. Masoom and M. Yaqoob, Production of phosphatidate by immobilized phospholipase-D, Process Biochemistry, 30 (1995) 701-4.

9.         M. Yaqoob, G. Hussain and M. Masoom, Determination of phosphoryl-choline with an alkaline phosphatase reactor using flow injection analysis, J. Chem. Soc. Pak., 17 (1995) 25-28.

10.       M. Anwar, M. Hashim, M. Yaqoob and M. Masoom, Enzymatic determination of electrophoretically separated LDL-cholesterol from sera of cardiac patients, J. Chem. Soc. Pak., 17 (1995) 40-42.

11.       G. Hussain, M. Yaqoob, A. Nabi and M. Masoom, Determination of uric acid and xanthine/hypoxanthine in blood using immobilized enzymes in flow injection analysis, J. Chem. Soc. Pak., 17 (1995) 109-12.

12.       A. Nabi, M. Yaqoob and S. Khatoon, Determination of lead in aqueous system by flow injection analysis coupled with spectrophotometer, J. Chem. Soc. Pak., 18 (1996) 25-28.

13.       A. Nabi, A. Rashid and M. Yaqoob, Flow injection analysis of hydrogen peroxide with peroxyoxalate chemiluminescence detection, J. Chem. Soc. Pak., 18 (1996) 211-14.

14.       A. Nabi and M. Yaqoob, Determination of vanadium (V) by flow injection analysis with spectrophotometric detection, J. Chem. Soc. Pak., 18 (1996) 220-22.

15.       A. Nabi, A. Rashid and M. Yaqoob, Chemiluminescent determination of cholesterol by flow injection analysis with immobilized cholesterol oxidase, Anal. Lett., 29 (1996) 2281-88.

16.       M. Yaqoob, A. Nabi, I. Hafeez, S. A. Ali and M. Masoom, Enzymatic spectrophotometric determination of glycerol-3-phosphate and glycerophosphorylcholine, Anal. Lett., 30 (1997) 61-68.

17.       M. Yaqoob, A. Nabi and M. Masoom, Flow injection Chemiluminescent determination of glycerol-3-phosphate and glycerophosphorylcholine using immobilized enzymes, J. Biolumin. & Chemilumin, 12 (1997) 1-5.

18.       M. Yaqoob, A. Nabi and M. Masoom, Chemiluminecent assays for choline and phospholipase-D using flow injection system, J. Biolumin. & Chemilumin, 12 (1997) 135-40

19.       M. Yaqoob, A. Nabi, A. Wahab, M. I. Kasi and M. Masoom, Spectrophoto-metric determination of ammonium ions in soil and water samples by flow injection analysis, J. Chem. Soc. Pak., 19 (1997) 313-16.

20.       M. Yaqoob, A. Nabi and M. Masoom, Enzymatic transesterification between sugar alcohol and oils, J. Chem. Soc. Pak., 19 (1997) 210-13.

21.       S. A. Ali, J. Iqbal, Y. K. Nabeel, M. Yaqoob , M. Asifullah, B. Ahmed,  I. Tareen and M. Masoom, New horizons in leishmania cultivation, J. College Physicians and Surgeons Pak., 7 (1997) 53-57.

22.       S. A. Ali, Y. K. Nabeel, Sher Ahmed, Fouzia S. Rehmani, M. Yaqoob and M. Masoom, Studies on the photometric estimation of citrate in urine, Pak. J. Biol. Sci., 1 (1998) 326-28.

23.       M. Yaqoob, A. Nabi, A. Wahab, Amna Bano, S. H. Shah and A. K. K. Achakzai, Spectrophotometric determination of sulphide using flow injection analysis, J. Chem. Soc. Pak., 20 (1998) 214-16.

24.       A. Nabi, M. Yaqoob and M. Anwar, Bioluminescence and chemiluminescence monitoring a flow injection analysis, Laboratory Robotics and Automation, 11 (1999) 91-96.

25.       M. Yaqoob, A. Nabi and M. Masoom, Flow injection spectrophotometric determination of choline using immobilized enzymes, J. Chem. Soc. Pak., 21 (1999) 34-38.

26.       M. Yaqoob, A. Nabi and M. Masoom, Bovine pancreatic lipase: Purification and applications in immobilized form for transesterification reactions, J. Chem. Soc. Pak., 21 (1999) 114-19     

27.       M. Yaqoob, A. Nabi,  M. A .Siddiqui, A. Waseem, A. K. K. Achakzai, A. Saeed, and M. Masoom, Enzymatic procedures for determination of phospholipids, J. Chem. Soc. Pak., 23 (2001) 171-75.

28.       M. Yaqoob, A. Nabi and M. Masoom, Bioconversion of phosphatidyl-choline to phosphatidylserine using immobilized enzyme mini-columns, Process Biochemistry, 36 (2001) 1181-85.

29.       M. Yaqoob, A. Nabi, Flow injection method for the determination of serine using immobilized enzyme, Talanta, 55 (2001) 1181-86.

30.       A.K.K. Achakzai, S. A. Kayani, M. Yaqoob and A. Nabi, Effect of fertilizer inoculation and sowing time on the uptake of P, K, and Na content of field grown mature soybean seeds, Online J. Biol. Sci., 2/12 (2002) 789-792.

31.     M. Yaqoob and A. Nabi, Flow injection chemiluminescent assays for glycerol and triglycerides using coimmobilized enzymes reactor, Luminescence 18/2 (2003) 67-71.

32.      A.K.K. Achakzai, S. A. Kayani, M. Yaqoob and A. Nabi, Effect of fertilizer,  inoculation and sowing time on free amino acids and mineral nitrogen content of field grown mature soybean seeds, Asian J. Plant Sciences 2/1 (2003) 132-138.

33.      A.K.K. Achakzai, S. A. Kayani, M. Yaqoob and A. Nabi, Effect of fertilizer and inoculation on lipase and urease activity of mature soybean cv. Williams-82 seeds, Asian J. Plant Sciences, 2/9 (2003) 692-95.

34       A.K.K. Achakzai, S. A. Kayani, M. Yaqoob, A. Nabi, and A. Waseem, Flow injection spectrophotometric determination of phosphate in soybean seed, soil and water samples, J. Flow Injection Analysis (Japan), 20/1 (2003) 57-59.

35.       A.K.K. Achakzai, S. A. Kayani, M. Yaqoob and A. Nabi, Chemical composition of soybean cv. William-82 seeds in pot culture experiments, J. Biological Sciences, 3/10 (2003) 882-91.

36.      M. Yaqoob, S. J. Ussher, A. Nabi, E. P. Achterberg and P. J. Worsfold,   Determination of Iron(II) in acidified seawater using flow injection with  chemiluminescence detection, J. Flow Injection Analysis (Japan), 20/2 (2003) 183-186.  

37.       M. Yaqoob, A. Nabi, A. Waseem and M. Masoom-Yasinzai, Determination of sulfite using an immobilized enzyme with flow injection chemiluminescence detection, Luminescence, 19/1 (2004) 26-30.

38.       M. Yaqoob, A. Nabi, and P. J. Worsfold, Determination of nanomolar concentrations of phosphate in freshwaters using flow injection with luminol chemiluminescence detection, Anal. Chim. Acta, 510/2 (2004) 213-18.

39.      M. Yaqoob, A. Nabi and P. J. Worsfold, Determination of silicate in freshwaters  using flow injection with luminol chemiluminescence detection, Anal. Chem. Acta, 519/2 (2004) 137-42.

40.      L. Rishi, M. Yaqoob, A. Nabi and M. Masoom, Flow injection procedure for determination of NAD+ using immobilized enzyme with spectrophoto-metric detection, J. Chem. Soc. Pak. 26 (2004) 367-71.

41.      A. Waseem, M. Yaqoob and A. Nabi, Determination of iron in blood serum using flow injection with luminol chemiluminescence detection, Luminescence, 19 (2004) 333-38.

42.       A. Waseem, A. Nabi and M. Yaqoob, Biotechnological potential of Ru(bpy)32+ using flow injection analysis, J. Applied & Emerging Sciences (BUITMS, Pak.), 1 (2004) 65-71.

43.       S. J. Ussher, M. Yaqoob, Eric. P. Achterberg, A. Nabi and P. J. Worsfold, Effect of model ligands on the determination of Fe(II) in natural waters using flow injection with luminol chemiluminescence detection, Anal. Chem., 77/7 (2005) 1971-78.

44.      M. Yaqoob, M Anwar and A. Nabi, Determination of phosphate in freshwaters using flow injection with immobilized enzyme and chemiluminescence detection, Int. J. Environ. Anal. Chem., 85/7 (2005) 451 - 59.

45.      L. Rishi, M. Yaqoob, A. Nabi and M. Masoom, Chromatographic removal of product in-line with immobilized enzyme bioreactor for the synthesis of NADH, J. Chem. Soc. Pak., 27/1 (2005) 59 – 64.

46.      M. Yaqoob, A. Waseem and A. Nabi, Determination of total iron using flow injection with acidic potassium permanganate chemiluminescence detection, J. Anal. Chem. Sept. (2005) accepted.

47.      M. Anwar, M. Yaqoob, A. Waseem and A. Nabi, Determination of ATP in clinical blood samples for quantitation of   bacteria, J. Chem. Soc. Pak., Sept. (2005) accepted.

48.      M. Anwar, M. Yaqoob and A. Nabi, Determination of adenosine 5’- triphosphate by flow injection with luminol chemiluminescence detection using immobilized alkaline phosphatase enzyme, J. Chem. Soc. Pak., Sept. (2005) accepted.

49.     M. Yaqoob, A. Waseem and A. Nabi, Determination of iodide using flow injection with acidic potassium permanganate chemiluminescence detection, Intern. J. Environ. Anal. Chem., Aug. (2005) submitted.

50.     A. Waseem, M. Yaqoob and A. Nabi, Determination of thyroxine in pharmaceuticals using flow injection with luminol Chemiluminescence detection, Luminescence, Sept. (2005) submitted.

51.     A.Waseem, M. Yaqoob and A. Nabi, Flow injection determination of thyroxine in pharmaceutical preparations using Ru(bpy)32+ - NADH system with chemiluminescence detection, J. Pharm. Biomed. Analysis, Sept. (2005) submitted.

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